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Instructions: Genetic Marker
Database: An excellent source for
listings of genetic markers, phenotypic traits affected, mapping data, and
references is: Holloway, B.W., U. Rohmling and B. Tummler, 1994. Genomic
mapping of Pseudomonas aeruginosa PAO. Microbiology 140:
2907-2929. We have adapted Table
1, Markers located on the genetic and physical maps of P. aeruginosa PAO,
from this paper to a database that you can search. The genetic and
physical map locations that the search tool will retrieve correspond to
Figures 1 & 2 from the paper. There are two ways
to search the genetic marker database: 1. Genetic Marker
Search: Similar to searching
the strain database by genotype, the more specific you are the less matches
you will see. For example, searching for "met" will yield more
matches than searching for "met-28". You will get a listing of all
markers known at the time of publication of the paper. If you get no matches
on your first attempt, try using only the three letter marker designation or
searching by phenotype (see below). Only enter one marker designation at a
time in the search field. The database is searching for any string of
characters containing your search entry, and the chances of you getting the
marker designations, numbers, and spacing of more than one genotype exactly
the same as the database entry are very slight. 2. Phenotype Keyword
Search: If you are looking for
the markers associated with a particular phenotypic trait, this search may
help you find them. It searches for the characters you enter anywhere
in the "Phenotypic Trait Affected" field of the genetic marker
database. It is best to enter single words, because it is hard to mimic the
ordering, spacing and punctuation of the actual database text. If
you get no results on your first attempt, try reducing the amount of
information in the search box and resubmitting your search. How to use results: When you view the
matches to each of your searches, you will be given the opportunity to search
the strain and cosmid databases for that marker. The marker designations in
the table of results are links to these search tools. Simply click on
the marker designation, and you will be taken to a page where you can choose
to search either for strains or cosmids. For more information on
searching the strain and cosmid databases, please return to the main instructions page and follow the links to the
appropriate instructions, or choose a link below. If you have difficulty
searching the database or submitting your request, please feel free to e-mail
your request to the curator at Pseudomonas@mail.ecu.edu. You can
also mail or fax requests or comments to:
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