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Instructions: Strain database: 1.
With
a few exceptions, all strains available from this website are derived from
PAO1. Strain designations are PAO, PFB and PRP. You may also find a couple of
prototrophic Pseudomonas putida strains with the designation PPN. You
can direct questions about other strains of interest to the curator by using
the comments page. 2.
Strain
numbers need to be entered in a very specific way to successfully search the
database. All of the numerical designations must be converted to 4 digits by
adding zeros.
3.
When
searching by genotype, the more specific you are the less matches you will
see. For example, searching for "met" will yield more matches than
searching for "met-28". You will get a listing of all strains with
this genotype and strains with this genotype and other genetic markers. If
you get no matches on your first attempt, try using only the three letter
marker designation. Only enter one marker designation at a time in the search
field. The database is searching for any string of characters containing your
search entry, and the chances of you getting the marker designations,
numbers, and spacing of more than one genotype exactly the same as the
database entry are very slight. An
excellent source for listings of genetic markers, phenotypic traits affected,
mapping data, and references is: Holloway, B.W., U. Rohmling and B. Tummler,
1994. Genomic mapping of Pseudomonas aeruginosa PAO. Microbiology
140: 2907-2929. How to use results: When you view the
matches to each of your searches, you will be given the opportunity to submit
requests for each of the strains. The "Availability" column of the
report chart allows you to check a box to add an available strain to your
request. Some strains indicate "Unavailable". These strains are left
in the database for historical/informational purposes only. No viable
cultures of these strains are known to exist at the Pseudomonas Genetic Stock
Center. Other strains indicate
"Not at ECU". These strains are not available from the ECU site and
must be shipped to us from Monash University in Australia. Monash is no
longer able to routinely provide strains for the Pseudomonas Genetic Stock
Center. Each request for these strains will be evaluated on a case by case
basis, but at this time it is highly unlikely that we will be able to acquire
any strain bearing that designation. If you check boxes to
request available strains you will then proceed to the request form, by
clicking on the "Request Checked Strains" button, to mail your
request to the collection curator. At this point you can also reduce your
list of requested strains. Please fill out this form completely and submit it
to have your request processed. After receiving your request, we may write or
call for more information. Please allow us several days to ship your request
to you. If you are working at a non-academic, for-profit facility, a small
fee of $25.00 per strain is required to cover our operating and shipping
expenses. An invoice will be sent in your shipment for you to submit to your
purchasing or Accounts Payable Department. If you have difficulty
searching the database or submitting your request, please feel free to e-mail
your request to the curator through the comments page,
or by writing to Pseudomonas@mail.ecu.edu.
You can also mail or fax requests or comments to:
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